Nhanganyaya

Mumazuva ano biomedical uye makemikari marabhoritari,anoraswa tsika machubhu anoshandiswa zvakanyanya musero tsika, kemikari maitiro, chengetedzo yemuenzaniso uye mamwe mabasa akakosha.Kukosha kwavo muchiitiko chekuedza hakugone kuregererwa. Sezvo aya machubhu etsika ari kudhumana zvakanangana nemasampuli ekuyedza, zvinhu zvawo, saizi, kuvharwa uye kunyangwe akavharwa kana kuti kwete zvichave nemhedzisiro yakakosha pamhedzisiro yekuyedza. Kusarudzwa kwakashata kunogona kutungamirira mukusvibiswa-kusvibiswa, kurasikirwa kwemuenzaniso kana kuedza kwekutsvaga data, nokudaro kukanganisa kurongeka uye kuberekazve kwechidzidzo.

Mhando Dzakakura dzeTsika Dzinoraswa Tubes

Kune huwandu hwakasiyana hwekuraswa hutsika machubhu, uye vaongorori vanofanirwa kusarudza mhando yakakodzera zvinoenderana nechinangwa chekuyedza, nharaunda yekushanda uye hunhu hwemasampuli. Iwo akaiswa muzvikamu zvitatu zvinotevera: zvinhu, kugona uye basa rinokosha:

1. Kuronga maererano nezvinhu

Anoraswa tsika machubhu akagadzirwa akasiyana zvinhu anosiyana mukupikiswa kwekushisa, kugadzikana kwemakemikari uye optical zvimiro:

• Polypropylen+
• Polystyrene: kujeka kwepamusoro, kuri nyore kuona iyo mvura uye sero mamiriro, inowanzoshandiswa mukuedzwa kwemaziso, asi kwete yakanyanya tembiricha kuramba, kazhinji haigone kushandiswa autoclaving.
• Girazi tsika machubhu: Kunyange zvazvo ichigona kushandiswazve uye yakagadzikana mumakemikari, inodhura, inoda kumwe kucheneswa nekuita stereolization, uye inopa njodzi inogona kuitika yekusvibiswa.

2. Kurongeka nekugona

Zvichienderana nehuwandu hwemuenzaniso unodiwa pakuedza, huwandu hwemachubhu ehutsika hunotangira kubva kudiki kusvika kuhombe mavhoriyamu:

• Microcentrifuge tubes: inowanzoshandiswa kugovera sampuli, centrifugal precipitation, DNA/RNA extraction uye mamwe maoperation.
• Standard tsika machubhu: iyo inonyanya kushandiswa simba murabhoritari, inokodzera sero tsika, kusanganisa kuita, kuchengetedza sampuli uye zvimwe zvinangwa.
• Machubhu makuru ehutsika: inokodzera hukuru hwesero tsika kana huwandu hukuru hwekugadzirisa mhinduro.

3. Kurongeka nebasa rinokosha

Iwo machubhu anowanikwa aine akasiyana ekuwedzera maficha kuti asangane nezvinodiwa zvekuedza:

• Aseptic Pre-sterilized Tubes: fekitori yakasvibiswa ne gamma radiation kana autoclaving, yakakodzera kuyedza nepamusoro aseptic zvinodiwa.
• Ne cartridge cap: inobvumira kuchinjana kwegasi, inokodzera tupukanana kana maseru mitsara inoda aeration uye inodzivirira kusvibiswa kubva kune exogenous masosi.
• Low Temperature Resistant Tubes: inogona kushandiswa zvakachengeteka mu -80 ℃ kana kunyange mvura nitrogen nharaunda, inokodzera kwenguva yakareba yakaderera tembiricha kuchengetedza kwebiological samples.
• Vakapedza kudzidza / vasina kudzidza machubhu: Machubhu akapedza kudzidza ari nyore kukurumidza kufungidzira uye kugovera vhoriyamu yemvura kuti uwedzere kushanda kwekuyedza.

Zvinhu Zvikuru Mukusarudza Tsika Tubes

Mukuita kwekuyedza dhizaini nekushanda, kusarudzwa kweakakodzera anoraswa tsika machubhu kwakakosha kuti ive nechokwadi chekuyedza kushanda uye huchokwadi hwemhedzisiro. Vatsvakurudzi vanofanira kunyatsoongorora zvinhu zvakati zvakakosha:

1. Mhando yekuedza

Zvinodiwa zvemachubhu etsika zvinosiyana zvakanyanya kubva pakuyedza kusvika pakuyedza uye zvinofanirwa kusarudzwa zvinoenderana nezviri mukati mekushanda uye nharaunda yekuyedza:

• Nezvesero tsika: zvinodikanwa zve sterility zvakanyanyisa kukwirira, uye kushandiswa kwemachubhu asina kuberekwa pre-sterilized ane kufema cartridge caps kuve nechokwadi chekuchinjana gasi kunokurudzirwa.
• PCR / molecular biology miedzo: inoda kuve isina DNA enzymes, RNA enzymes uye pyrogen-isina tubing, kazhinji inoshandisa yakachena-giredhi polypropylene tubing.
• Kuchengetedza kwakadzika kwekushisa: Machubhu ane yakanaka yakaderera tembiricha kuramba kunofanira kushandiswa kudzivirira lysis panguva yakaderera tembiricha.

2. Muenzaniso maitiro

Iyo physicochemical zvimiro zvemuenzaniso zvinopesvedzera zvakananga kusarudzwa kwezvinhu uye kushanda kwemaitiro emachubhu etsika:

• Liquid kana solid samples: inosarudza vhoriyamu inodiwa uye chimiro chechubhu orifice.
• Acid kana alkaline samples: Sampuli dzinoparadza zvakanyanya dzinoda zvinhu zvinodzivirira kemikari kudzivirira deformation yechubhu kana kusvibiswa.
• Kunzvenga chiedza here: masampula-anonzwa anofanirwa kusarudza amber kana opaque zvinhu tsika chubhu, kuitira kudzivirira kuparara kwemifananidzo.

3. Sterilization zvinodiwa

Iko kudiwa kwekuita sterilization uye rudzi rwesterilization inoshandiswa ndiyo mifungo yakakosha mukusarudzwa kwechubhu:

• Pre-sterilization vs. self-sterilization: fekitori pre-sterilized zvigadzirwa zvakakodzerwa ne-high-throughput miedzo, kuchengetedza nguva; kana rabhoritari iine autoclave sterilization zvishandiso, unogona kusarudza PP tubing inogona kuvharirwa.
• Sterilization kuenderana: Semuenzaniso, zvinhu zvePS hazvina kukodzera autoclaving uye ndeyekushandisa kamwe chete.

4. Kugarisana

Iwo machubhu anofanirwa kunyatso kuchinjirwa kumidziyo yerabhoritari kuti ive nechokwadi chekushanda kwakanaka uye mhedzisiro yakavimbika:

• Centrifugal kugarisana: Machubhu anoshandiswa kuita centrifugation anofanirwa kuve akasimba muchimiro kuti akwanise kushingirira centrifugal masimba pakumhanya kukuru kwekutenderera.
• Automation kugarisana: Nezviedzo zvinoda kushandiswa kwemarobhoti epombi, otomatiki masisitimu ekuparadzira, nezvimwewo, saizi yakajairwa yechubhu inofanirwa kushandiswa.

5. Mutengo uye kuchengetedza

Kudzora zvine musoro kwemitengo uye mashandisirwo ezvishandiso zvakare chinhu chakakosha kufunga mukusangana nezvido zvekuyedza:

• Disposable vs. reusable: machubhu anoraswa ari nyore kubata uye kudzivirira kusvibiswa, akakodzera kune yakakwira-kupfuura miyedzo; reusable girazi machubhu akakodzera kuyedza kwekutanga nebhajeti shoma.
• Purchase scale: Kutenga kukuru kwevhoriyamu kunogona kuderedza mutengo weyuniti, yakakodzera kwenguva refu, mapurojekiti makuru; zvidiki-zvidiki, zvakagadziridzwa zvakatemwa zvinochinjika asi zvine mutengo wakakura.

Recommendations for Common Application Scenarios

Zvinoenderana nezvinodiwa zvemhando dzakasiyana dzekuyedza, anotevera anoraswa tsika machubhu anokurudzirwa kune akati wandei akajairwa maitiro ekushandisa, achivavarira kuvandudza mashandiro uye kuvimbika kwezviedzo:

1. Kurimwa kwemasero

• Yakakurudzirwa mhando: sterile polypropylene tsika machubhu ane inofema cartridge caps
• Chikonzero: Polypropylene zvinhu zvine yakanaka makemikari inertness uye biocompatibility, yakakodzera sero tsika fluid. Iyo cartridge yekuvharira inogona kuona inoshanda gasi kuchinjana, kudzivirira kusvibiswa kwehutachiona, uye kusangana nenharaunda yegasi inodiwa mukuwanda kwesero.

2. PCR/qPCR molecular realization

• Mhando dzakakurudzirwa: nuclease-isina, pyrogen-isina nyanzvi PCR machubhu kana microcentrifuge chubhu
• Chikonzero: Yakakwirira kuchena polypropylene machubhu, mushure mekurapa kwakasimba, anogona kunyatso dzivirira kusvibiswa kwemuenzaniso kana kusvibiswa, kuve nechokwadi chechokwadi uye chakavimbika mibairo yekukudza. Thin-wall dhizaini inokurudzirwa kunatsiridza kupisa kutamisa kushanda.

3. Kuchengetedza kwakadzika kwekushisa

• Yakakurudzirwa Type: Yakaderera Tembiricha Inodzivirira Polypropylene Tubing ine Anti-Freeze Cracking Dhizaini uye Screw Seal Cap
• Chikonzero: Aya machubhu anogona kushandiswa kuchengeta sampuli kwenguva yakareba pa -80 ° C kana kunyange mu nitrogen yemvura, uye chimiro chakakosha chinodzivirira chubhu kubva pakuputika uye sampuli yekudonha. Inokodzera kuchengetwa kwenguva refu kwemasero mitsara, sampuli dzeropa, mapuroteni kana nucleic acids.

4. Centrifuge

• Mhando dzakakurudzirwa: yakakwirira-kushivirira polypropylene machubhu, yakatenderera kana conical pasi dhizaini, ine centrifuge rotors
• Chikonzero: PP machubhu ane yakanaka centrifugal kuramba uye anogona kutsungirira yakakwirira centrifugal masimba pasina deformation kana kuputika. Iyo conical pasi inobatsira kuunganidza maseru kana kudonha nechepakati uye kuwedzera chiyero chekudzoreredza.

Zvakajairika Kukanganisa uye Nzira Dzekunzvenga Nadzo

Mukuita, kukundikana kwekuedza nekuda kwekusarudzwa kusina kunaka kwetsika tubes kunoitika nguva nenguva. Kukanganisa kwakawanda kwakajairika uye mazano anowirirana emhinduro akanyorwa pazasi kuti aonekwe nevaongorori:

1. Kusashandiswa zvisina kunaka kwezvinhu zvisingadziviriri tembiricha zve autoclaving